S: WHO – https://www.who.int/influenza/gisrs_laboratory/pcr_working_group/en/ (last access: 10 April 2020); NHS – https://www.nhs.uk/conditions/acute-lymphoblastic-leukaemia/diagnosis/ (last access: 10 April 2020).
N: 1. PCR: polymerase chain reaction.
2. Polymerase chain reaction (PCR), a technique used to make numerous copies of a specific segment of DNA quickly and accurately. The polymerase chain reaction enables investigators to obtain the large quantities of DNA that are required for various experiments and procedures in molecular biology, forensic analysis, evolutionary biology, and medical diagnostics.
3. PCR was developed in 1983 by Kary B. Mullis, an American biochemist who won the Nobel Prize for Chemistry in 1993 for his invention. Before the development of PCR, the methods used to amplify, or generate copies of, recombinant DNA fragments were time-consuming and labour-intensive. In contrast, a machine designed to carry out PCR reactions can complete many rounds of replication, producing billions of copies of a DNA fragment, in only a few hours.
4. The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
5. The method relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA.
S: 1. MW – https://www.merriam-webster.com/dictionary/PCR (last access: 10 April 2020). 2&3. EncBrit – https://www.britannica.com/science/polymerase-chain-reaction (last access: 10 April 2020). 4 & 5. TERMIUM PLUS – https://www.btb.termiumplus.gc.ca/tpv2alpha/alpha-eng.html?lang=eng&i=1&srchtxt=PCR&index=alt&codom2nd_wet=1#resultrecs (last access: 10 April 2020)
SYN: PCR, PCR method.
S: TERMIUM PLUS – https://www.btb.termiumplus.gc.ca/tpv2alpha/alpha-eng.html?lang=eng&i=1&srchtxt=PCR&index=alt&codom2nd_wet=1#resultrecs (last access: 10 April 2020)
CR: coronavirus, COVID-19, serological test.